The effect of pH within the kinetic constants for that hydrolysis of substrates by NagA was measured within an try to detect These purposeful groups from the substrate and active site which might be required to be in a particular point out of protonation. The log k
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m just isn't constrained through the association rate continual to the formation of your NagA-substrate sophisticated. These success (along with the improved charge of reaction of compound 6 relative to compound 1) are only consistent with the summary that NagA is proscribed by either the formation or breakdown on the tetrahedral intermediate.
Nāga because the lesser deity of earth and water is commonplace within the Hindu period of Indonesia, before the introduction of Islam.
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The precise role of the metal cofactor is of particular interest for NagA as the two crystal buildings accessible for the enzyme from E. coli